Elisa Reader And Washer Principle

The enzyme activity is measured using a substrate that changes color when modified by the enzyme.

Elisa reader and washer principle.

The test is done in an elisa plate also known as a 96 well plate or microplate. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. Elisa stands for enzyme linked immunosorbent assay. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present.

And a plate reader software is used to plot standard curves and calculate results. The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically. The elisa reader reads the plate. Readers need to have appropriate filter 650 nm and 450 nm.

What an elisa reader does. The illustration below shows a workflow for a typical sandwich elisa assay. Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. Elisa is a plate based assay technique.

Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay. It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates.